Protocols

All protocols

Complete, versioned methods. Filter by area, open a protocol, tick steps off as you work, and print a bench copy.

CTAB extraction of genomic DNA from plant leaf tissue

CTAB-based extraction of high-molecular-weight genomic DNA from 50–100 mg of leaf tissue, with recipes, critical steps and quality-control targets.

About 2.5 hours
Beginner
v1.0 · 25 October 2026

Transformation

Floral dip transformation of Arabidopsis thaliana

Agrobacterium floral dip for stable Arabidopsis transformation, from plant preparation and bacterial culture to T1 selection and confirmation.

About 8–10 weeks seed to confirmed T1
Intermediate
v1.0 · 25 October 2026

Genome editing

Genotyping CRISPR edits in plants by PCR, Sanger sequencing and trace decomposition

Amplify the target site, Sanger-sequence it, and decompose the trace with TIDE or ICE to call indel genotypes in T0 and T1 plants, with rules for calling and confirming chimeras.

About 2 days including sequencing turnaround
Intermediate
v1.0 · 25 October 2026