plantbiomethodsPlant biology methods
Protocols written to be repeated.
Bench-tested methods for plant molecular biology, transformation and genome editing. Every protocol gives complete recipes, marks the steps that make or break it, explains why each step is there, and cites the primary paper it comes from.
- 3complete protocols, each versioned and dated
- 5method areas, from nucleic acids to genome editing
- ✓Tick steps as you go, and print a clean bench copy
Method areas
Nucleic acids
DNA and RNA extraction, quality control and quantification from plant tissue.
1 protocolTransformation
Getting constructs into plants: Agrobacterium, floral dip, hairy roots and tissue culture.
1 protocolGenome editing
CRISPR design, delivery and, above all, finding out what edit you actually made.
1 protocolMutagenesis
Chemical and physical mutagenesis, population development and mutant screening.
In preparationStaining and imaging
Reporter assays, histochemical stains and microscopy of plant cells and tissues.
In preparationProtocols
All protocols →CTAB extraction of genomic DNA from plant leaf tissue
CTAB-based extraction of high-molecular-weight genomic DNA from 50–100 mg of leaf tissue, with recipes, critical steps and quality-control targets.
Beginner
v1.0 · 25 October 2026
Floral dip transformation of Arabidopsis thaliana
Agrobacterium floral dip for stable Arabidopsis transformation, from plant preparation and bacterial culture to T1 selection and confirmation.
Intermediate
v1.0 · 25 October 2026
Genotyping CRISPR edits in plants by PCR, Sanger sequencing and trace decomposition
Amplify the target site, Sanger-sequence it, and decompose the trace with TIDE or ICE to call indel genotypes in T0 and T1 plants, with rules for calling and confirming chimeras.
Intermediate
v1.0 · 25 October 2026
How every protocol is written
Complete recipes
Every buffer has its composition, amounts for a working volume, and how to store it. No "standard buffer" left undefined.
Why, not just what
Each protocol opens with the principle behind it, so you can adapt it to a new species or tissue instead of following it blind.
Critical steps marked
Steps that decide success are flagged, and safe pause points are marked so you know where you can stop for the day.
Troubleshooting from experience
A table of what goes wrong, why, and what to change, written from the failures that actually happen at the bench.
Primary sources
Each method cites the paper it comes from, with a DOI, so you can check the original conditions.
Versioned
Protocols carry a version number and update date, and changes are logged, so a citation always points to a fixed text.